Chronic arsenic exposure and microbial drug resistance.

Resistance to antimicrobial drugs represents one of the greatest threats to the control of infectious diseases and is a particular problem in treating diseases caused by parasitic protists. These pathogens are of enormous medical importance, causing diseases such as malaria, toxoplasmosis, trypanosomiasis, and leishmaniasis. In the absence of effective vaccines, the control of these diseases is critically dependent on drug therapies that are often undermined by poor compliance or overuse in communities with struggling health care systems and endemic poverty. The development and spread of resistance to the antimalarial drug chloroquine from the 1950s onwards constitutes a spectacular example of drug failure caused by overuse. Similarly, the development of antimonial-resistance in Leishmania donovani in India in the 1970– 1980s had all of the hallmarks of long-term misuse (1). L. donovani is the causative agent of visceral leishmaniasis (VL) or Kala-Azar, which kills many thousands of people in India each year. However, in PNAS, Perry et al. (2) provide compelling evidence that Leishmania-acquired antimonial resistance in India may be attributable, at least in part, to increased arsenic contamination of the drinking water. These findings highlight how environmental factors can contribute to the emergence of microbial drug resistance and have implications for drug development. Leishmania are insect-transmitted protist parasites that cause a spectrum of diseases affecting more than 12 million people worldwide. Depending on the Leishmania species involved (there are 20 that infect humans) and the host immune status, symptoms can range from localized, self-resolving skin lesions to deadly visceral infections where parasites target immune cells in the liver and spleen (3). VL is a particular problem in the eastern Indian state of Bihar as a result of widespread poverty and hyperendemicity of L. donovani, which is transmitted from human to human by a sandfly vector. Pentavalent antimonials, such as pentostam, were first used to treat VL in the 1920s and deployed successfully for many decades. However, in the 1970s resistance was noted during epidemics caused by the cessation of vectorcontrol programs in Bihar. Drug doses and length of treatments were progressively increased (from 6 d to 30 d) in the face of falling treatment success rates, and antimonials were finally replaced by lipid amphotericin B and miltefosine in 2002. Although the development of antimonial resistance in Bihar is often attributed to misuse, the fact that similar levels of resistance have not been observed in other parts of the world suggests that other factors unique to Bihar State were in play. Perry et al. noted that the development of antimonial resistance in Bihar State in the 1970s coincided with increased arsenic contamination of drinking water as a result of the widespread insertion of shallow tube wells for the provision of clean drinking water (4). Arsenic, like antimony, belongs to group 15 of the periodic table and the two metalloids share many chemical properties. In fact, organic arsenic formulations have previously been used to treat leishmaniasis and the arsenicals remain one of the mainstay therapies for treating human African trypanosomiasis (5). Perry et al. hypothesized that long-term exposure and subsequent adaptation of L. donovani to sublethal levels of arsenic in their human hosts may have led to cross-resistance to antimonials (4). In support of this hypothesis they now show that antimonial-susceptible L. donovani strains repeatedly passaged in mice exposed to low levels of arsenic in their drinking water (comparable to those observed in Bihar) evolved strong resistance to pentostam. The acquisition of antimonial resistance was associated with elevated levels of arsenic in the liver and was stable after multiple passages through arsenic-free animals. Significantly, antimonial-resistant parasites seem to be more pathogenic than antimonial-susceptible lines, even in the absence of drug selection (2, 6), suggesting that antimonial/arsenic cross-resistant parasites might persist and be transferred to neighboring areas where arsenic contamination is not a problem. This finding contrasts with the more common situation where the development of drug resistance is associated with a loss of fitness in the mammalian host. For example, in some areas where chloroquine use for malaria treatment has been discontinued, chloroquine-sensitive strains have gradually replaced the resistant strains, allowing chloroquine to be effectively used again (7). A comparable scenario seems, unfortunately, less likely for antimonial-resistant Leishmania, particularly if environmental exposure to arsenic persists. Pentavalent antimonials (Sb) are transported across the host and parasite membranes and subsequently reduced to the bioactive Sb form in either the host cell or intracellular parasite stages (8) (Fig. 1). Sb is thought to primarily target parasite enzymes Fig. 1. Mechanisms of Leishmania resistance to antimonials that may be induced by chronic exposure to arsenic. Accumulation of arsenic (As) in the liver, one of the major organs targeted by L. donovani, leads to cross-resistance to related antimony (Sb). Resistance mechanisms include increased efflux or intracellular sequestration of Sband Sb-trypanothione (TSH) conjugates, as well as activation of anti-inflammatory cytokines (such as IL-10) that promote parasite growth.